Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana.
Identifieur interne : 000751 ( Main/Exploration ); précédent : 000750; suivant : 000752Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana.
Auteurs : Mateusz Wydro [Pologne] ; Edward Kozubek ; Przemysław LehmannSource :
- Acta biochimica Polonica [ 0001-527X ] ; 2006.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), Acétophénones (pharmacologie), Agrobacterium tumefaciens (génétique), Cysteine endopeptidases (génétique), Cysteine endopeptidases (physiologie), Expression des gènes (effets des médicaments et des substances chimiques), Expression des gènes (génétique), Extinction de l'expression des gènes (physiologie), Feuilles de plante (génétique), Modèles génétiques (MeSH), Plasmides (génétique), Potyvirus (génétique), Protéines virales (génétique), Protéines virales (physiologie), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Tabac (génétique).
- MESH :
- effets des médicaments et des substances chimiques : Expression des gènes.
- génétique : ARN des plantes, Agrobacterium tumefaciens, Cysteine endopeptidases, Expression des gènes, Feuilles de plante, Plasmides, Potyvirus, Protéines virales, Protéines à fluorescence verte, Tabac.
- métabolisme : Protéines à fluorescence verte.
- pharmacologie : Acétophénones.
- physiologie : Cysteine endopeptidases, Extinction de l'expression des gènes, Protéines virales.
- Modèles génétiques.
English descriptors
- KwdEn :
- Acetophenones (pharmacology), Agrobacterium tumefaciens (genetics), Cysteine Endopeptidases (genetics), Cysteine Endopeptidases (physiology), Gene Expression (drug effects), Gene Expression (genetics), Gene Silencing (physiology), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Models, Genetic (MeSH), Plant Leaves (genetics), Plasmids (genetics), Potyvirus (genetics), RNA, Plant (genetics), Tobacco (genetics), Viral Proteins (genetics), Viral Proteins (physiology).
- MESH :
- chemical , genetics : Cysteine Endopeptidases, Green Fluorescent Proteins, RNA, Plant, Viral Proteins.
- chemical , metabolism : Green Fluorescent Proteins.
- chemical , pharmacology : Acetophenones.
- drug effects : Gene Expression.
- genetics : Agrobacterium tumefaciens, Gene Expression, Plant Leaves, Plasmids, Potyvirus, Tobacco.
- chemical , physiology : Cysteine Endopeptidases, Gene Silencing, Viral Proteins.
- Models, Genetic.
Abstract
Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 microM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.
PubMed: 16582986
Affiliations:
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(génétique)</term><term>Potyvirus (génétique)</term><term>Protéines virales (génétique)</term><term>Protéines virales (physiologie)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Tabac (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cysteine Endopeptidases</term><term>Green Fluorescent Proteins</term><term>RNA, Plant</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Acetophenones</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Expression des gènes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Gene Expression</term><term>Plant Leaves</term><term>Plasmids</term><term>Potyvirus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Agrobacterium tumefaciens</term><term>Cysteine endopeptidases</term><term>Expression des gènes</term><term>Feuilles de plante</term><term>Plasmides</term><term>Potyvirus</term><term>Protéines virales</term><term>Protéines à fluorescence verte</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acétophénones</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cysteine endopeptidases</term><term>Extinction de l'expression des gènes</term><term>Protéines 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Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 microM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16582986</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>27</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0001-527X</ISSN><JournalIssue CitedMedium="Print"><Volume>53</Volume><Issue>2</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>Acta biochimica Polonica</Title><ISOAbbreviation>Acta Biochim Pol</ISOAbbreviation></Journal><ArticleTitle>Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana.</ArticleTitle><Pagination><MedlinePgn>289-98</MedlinePgn></Pagination><Abstract><AbstractText>Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 microM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wydro</LastName><ForeName>Mateusz</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kozubek</LastName><ForeName>Edward</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Lehmann</LastName><ForeName>Przemysław</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>04</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Poland</Country><MedlineTA>Acta Biochim Pol</MedlineTA><NlmUniqueID>14520300R</NlmUniqueID><ISSNLinking>0001-527X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000098">Acetophenones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>866P45Y84S</RegistryNumber><NameOfSubstance UI="C051667">acetosyringone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D003546">Cysteine 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PubStatus="revised"><Year>2006</Year><Month>02</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>03</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>4</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>12</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>4</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16582986</ArticleId><ArticleId IdType="pii">20061203</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pologne</li></country></list><tree><noCountry><name sortKey="Kozubek, Edward" sort="Kozubek, Edward" uniqKey="Kozubek E" first="Edward" last="Kozubek">Edward Kozubek</name><name sortKey="Lehmann, Przemyslaw" sort="Lehmann, Przemyslaw" uniqKey="Lehmann P" first="Przemysław" last="Lehmann">Przemysław Lehmann</name></noCountry><country name="Pologne"><noRegion><name sortKey="Wydro, Mateusz" sort="Wydro, Mateusz" uniqKey="Wydro M" first="Mateusz" last="Wydro">Mateusz Wydro</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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